![]() ![]() ![]() Along with increasing amounts of blood, the frequency of false pathological protein results increased. In contrast, already a low blood contamination (2,500 erythrocytes/μl CSF) led to false pathological results of total protein and albumin. Results: Cytological examination revealed no evidence of erythrophages or siderophages in vitro. Blood contamination was simulated in vitro by adding different amounts of blood ending up with five different samples containing erythrocyte counts of 2,500, 5,000, 7,500, 10,000, and 20,000 per μl CSF. In the third measurement series, the effects of artificial blood contamination on CSF protein results were investigated. In both types of experiments, CSF including blood was incubated for 24 h and for 72 h at room temperature or at 4☌. (2) CSF was investigated when blood contamination occurred during a traumatic lumbar puncture. Methods: Two measurement series were performed in order to investigate the role of artificial blood contamination on the possible development of erythrophages and siderophages in the CSF: (1) blood contamination was simulated in vitro by adding blood into the CSF. Furthermore, there is no consensus about the acceptable amount of artificial blood contamination on CSF protein results. It is controversially discussed if phagocytosis of erythrocytes which can be found in the CSF after subarachnoid hemorrhage can also develop in vitro in the presence of artificial blood contamination. Background: Blood contamination due to traumatic lumbar puncture presents a diagnostic pitfall in cerebrospinal fluid (CSF) analysis. ![]()
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